Mass Spectrometry of Glycoproteins
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內容簡介
1. Introduction to Glycosylation and Mass Spectrometry
Steven M. Patrie, Michael J. Roth and Jennifer J. Kohler
Part I - Enrichment and Isolation Methods
2. Tandem Lectin Weak Affinity Chromatography (LWAC) for Glycoprotein Enrichment
Zhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller
3. CSC Technology - Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells
Andreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid
4. Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment
Yawei Xu, Lijuan Zhang and Haojie Lu
5. Incorporation of Unnatural Sugars for the Identification of Glycoproteins
Balyn W. Zaro, Howard C. Hang, Matthew R. Pratt
6. Characterization of Membrane-associated Glycoproteins using Lectin Affinity Chromatography and Mass Spectrometry
Yashu Liu, Jintang He and David M. Lubman
7. Sialic Acid Capture-and-Release and LC-MSn Analysis of Glycopeptides
Jonas Nilsson and G繹ran Larson
Part II - Sample Preparation
8. In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass AnalysisEden P. Go, Kathryn R. Rebecchi, and Heather Desaire
9. Nano-HPLC-MS of Glycopeptides Obtained After Non-specific Proteolysis
Gerhild Zauner, Carolien A. M. Koeleman, Andr矇 M. Deelder and Manfred Wuhrer
Part III - Separation Methods
10. Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Chromatography Solid Phase Extraction (HILIC SPE)
Pia H繪nnerup Jensen, Simon Mysling, Peter H繪jrup and Ole N繪rregaard Jensen
11. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection
Alina D. Zamfir , Corina Flangea, Alina Serb3, Ana-Maria Zagrean4, Andreas M. Rizzi5 and Eugen Sisu
12. Structural Separations by Ion Mobility-MS for Glycomics and Glycoproteomics
Larissa S. Fenn and John A. McLean
Part IV - Quantitation Methods
13. Quantitative Analysis of Glycoprotein Glycans
Ron Orlando
14. Stable Isotope Labeling of N-glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometry-based Glycoproteomics
Hiroyuki Kaji and Toshiaki Isobe
15. Approaches for Site Mapping and Quantification of O-linked Glycopeptides
Peng Zhao, Stephanie H. Stalnaker, Lance Wells
16. Glycan Profiling: Label-free Analysis of Glycoproteins
Yoshinao Wada
Part V - Computational Tools
17. Introduction to Informatics in Glycoprotein Analysis
Kiyoko F. Aoki-Kinoshita
18. Software Tools for Glycan Profiling
Chuan-Yih Yu, Anoop Mayampurath, Haixu Tang
Part VI - Case Studies in Mass Spectrometry of Glycoproteins
19. Quantitative Characterization of Glycoproteins in Neurodegenerative Disorders using iTRAQ
Min Shi, Hyejin Hwang and Jing Zhang
20. Quantitative Proteomic Analysis of N-linked Glycoproteins in Human Tear Fluid
Lei Zhou and Roger W. Beuerman
21. Elucidation of N-glycosites Within Human Plasma Glycoproteins for Cancer Biomarker Discovery
Penelope Drake, Birgit Schilling, Brad Gibson and Susan Fisher
22. Characterizing the Glycosylation State of Therapeutic Recombinant Glycoproteins
Nicole Samuels, David Kates, Jun Liu, Joanne Severs
11. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection
Alina D. Zamfir , Corina Flangea, Alina Serb3, Ana-Maria Zagrean4, Andreas M. Rizzi
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