Protected Cultivation of Horticultural Crops
Protected cultivation, also known as controlled environment agriculture or greenhouse cultivation, is a modern agricultural practice used to grow horticultural crops under controlled environmental conditions. This method provides several advantages, including protection from adverse weather, pests, and diseases, as well as the ability to optimize growing conditions for maximum crop yield and quality. It typically utilizes greenhouse structures that allow growers to control various environmental factors such as temperature, humidity, light intensity, and CO2 levels, maintaining ideal conditions for plant growth. This cultivation method is suitable for various horticultural crops, including vegetables, fruits, flowers, and ornamental plants, enabling year-round production, disease and pest management, extended growing seasons, improved crop quality, and prolonged post-harvest freshness. At present, the protected cultivation of horticultural crops has evolved significantly, with a strong emphasis on sustainability.
Thin Film Microelectronic Devices and Circuits
This Special Issue comprises 10 original papers about recent advances in the research and development of thin-film microelectronic devices and circuits (TFMDCs). Specifically, three microelectronic devices are covered here, namely thin-film transistors (three papers), two terminal thin-film components (five papers), and optical devices (two papers).
Computational Approaches in Molecular Interaction Analysis
This book presents a curated collection of research articles from the Journal of Bioinformatics and Computational Biology, highlighting advances in protein-protein interaction-based computational methods for predicting protein complexes, host-pathogen interactions (HPIs), and protein functions. In Part I, it addresses the dynamic challenges of protein complex prediction using innovative techniques like GECA and IPC-RPIN, which integrate network topology, gene expression, and GO annotations to improve accuracy, especially for small complexes. Part II focuses on HPIs, reviewing computational approaches, predictive models, and tools that enhance understanding of infectious diseases, with a particular emphasis on machine learning applications. Part III delves into protein function prediction, employing PPI networks, multi-label methods, and iterative models that account for dynamic interactions. Collectively, these studies provide a comprehensive resource for advancing computational biology and understanding cellular and disease mechanisms.
QTL Mapping of Seed Quality Traits in Crops
This Special Issue "QTL Mapping of Seed Quality Traits in Crops" presents cutting-edge research on the genetic determinants of key seed quality traits, such as protein content, sugar accumulation, frost tolerance, seed longevity, and biochemical composition. Through advanced QTL mapping techniques, these studies provide critical insights into the genetic architecture of seed traits across major crops, including soybean, wheat, common bean, mustard, and Capsicum annuum.This collection showcases the integration of high-throughput genomics, phenotyping, and bioinformatics to identify candidate genes and molecular markers that can drive crop improvement. The findings in this issue are invaluable for plant geneticists, breeders, and agricultural scientists working to enhance seed quality, improve nutritional value, and increase resilience to environmental stresses.
Know thyself
In this popular science book, through dialogues with an artificial intelligence specialist, a physiologist presents the basic principles of the organism. For the first time, the organism is considered as a community of 220 types of selfish cells. All cells of the body (and there are about 50 trillion of them) compete for common resources. No cell "wants" to work for another, as this requires the expenditure of its limited energy. Nevertheless, the life products of some cell types have proven useful for others. Such causal chains between different types of cells (e.g., neurons and muscle cells, neurons and secretory cells), as a result of which the organism escaped predators or obtained food, were also useful to the organism. Evolution has preserved only those species whose cells had the ability to counteract the "despotism" of the brain and restore optimal metabolism. Man is healthy as long as biochemical and physiological multilevel mechanisms provide a compromise between effector cells and the main organizer of behavioral response - the brain. It is shown how slowly developing diseases of age are associated with disturbances in this compromise.
Fungi for optimum ligninolytic enzyme production in Chhattisgarh
Lignocellulose is the major component of plant cell wall which can be degraded by natural degraders such as Bacteria and Fungi. Lignin being amorphous in nature lacks stereo regularity. Despite of its resistant nature it has been reported to be degraded by Laccase, Lignin peroxidase and Manganese Peroxidase secreted by Bacteria and Fungi. It protects cellulose and Hemicellulose from enzymatic hydrolysis. Thus it is important to degrade lignin so that cellulose and hemicellulose are made available for degradation. Lignin is the only substance which can be completely degraded into water and Carbon dioxide by microbes. Thus microorganisms have the capacity to cycle lignin to humus. The enzymatic activity of the fungi (Aspergillus niger) as obtained was noted to be higher than the growth rate indicating that the isolated fungi have a great biotechnological potential. The fungi have high conversion rate and high de-polymerization efficiency.
Rat Lungworm Disease
The pathogenic nematode Angiostrongylus cantonensis is a leading global causative agent of eosinophilic meningitis in humans and other species. Clinically, this disease is known as neuroangiostrongyliasis but is colloquially recognized as rat lungworm disease. This disease has resulted in morbidity and mortality in humans and other accidental hosts, and the geographic range of this pathogen continues to increase through global expansion. The first five studies in this reprint provide updates on recent global research efforts and distribution, including studies from India, Brazil, Ecuador, reports of coinfections in Spain, and the genetic-based determination of global spread patterns of A. cantonensis. The other six studies evaluate potential mechanisms for prevention, such as creating barriers to decrease the mollusk infestation of crops; disease diagnostics applying both DNA and antibody-based methods; and current approaches to the pharmacological management of this disease using a multi-drug approach in humans, as well as investigating the effects on A. cantonensis of a commercially available antiparasitic currently used for controlling heartworm, fleas, and ticks in dogs. Guest Editor Susan I. Jarvi is a Professor in the department of Pharmaceutical Sciences, where she teaches Pharmaceutical Immunology and other infectious disease-related subjects at the Daniel K. Inouye College of Pharmacy at the University of Hawaii in Hilo, HI.
Quantum Mechanics
Quantum mechanics has brought a gigantic breakthrough to physics since its introduction more than a century ago. It has led to many discoveries at the microscopic level, initiated new pathways to unsuspected properties of matter, and produced new and amazing usable applications. This Special Issue of Symmetry is devoted to recent advances in theoretical developments in quantum mechanics. It is part of the global effort to provide a continuous supply of information to the research community. This volume gathers contributions in various directions and their contents are best described chronologically by their abstracts, which are hereafter listed for the convenience of the reader.
RSV Epidemiological Surveillance
Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory tract infection worldwide. Most children infected with RSV typically show mild respiratory symptoms. In newborns and infants, the typical clinical manifestations are bronchiolitis and pneumonia: the first is usually self-limiting, but accounts for a significant number of hospitalizations and admissions to the pediatric intensive care unit.Before the COVID-19 pandemic, RSV was typically described as a seasonal virus, characterized by a predictable epidemiological pattern, depending on the geographic area and climate. Due to restrictive measures adopted during the pandemic, many authors reported an initial reduction in RSV circulation, followed by an out-of-season surge of RSV as the measures adopted during the COVID-19 pandemic loosened. Epidemiological surveillance suggests that this trend in the epidemic wave affected not only children, but also adults, in particular the elderly. On the other hand, newly introduced prophylactic measures, such as nirsevimab administration to newborns and infants, are positively affecting the global picture. This Special Issue provides a comprehensive scenario of the epidemiological trend of RSV before, during, and after the COVID-19 pandemic. Furthermore, it represents a starting point for designing future preventive strategies to reduce RSV spread in both children and adults.
Integrated Technologies in Electrical, Electronics and Biotechnology Engineering
The conference was aimed to bring researchers, practicing engineers, faculty members and students from across the globe to a common platform to share their research ideas that would pave way to attain solution to various real time problems.
Moringa oleifera as an apoptosis inducer for colorectal cancer
Metal Ions in Biochemistry
The second edition of the book "Metal Ions in Biochemistry" deals exhaustively with the role of metal and non-metal ions in biochemical reactions, and also the effect of deficiency of metal ions, toxic effects of metal ions and use of metal complexes in therapeutics.
Impact of Environmental Factors and Management Practices on Bee Health
This compilation of twenty-three original articles and a communication underscores the urgency of using interdisciplinary approaches to safeguard bee health and ecosystem sustainability. The published articles explore complex factors affecting health, emphasizing the global honeybee and pollinator populations crisis. Key insights include Stressors on Bee Populations (pathogens, pests (e.g., V. destructor), pesticides, habitat loss, and climate change); Control Measures (studies highlighting effective methods such as queen caging and oxalic acid treatments for Varroosis control, essential oils as alternatives to synthetic acaricides, and advancements in diagnostic tools such as qPCR assays for diseases such as American foulbrood); the Impact of Environmental and Agricultural Practices (urbanization, pesticide exposure, and agricultural intensification affect bees differently, e.g., urban areas showed a higher pathogen prevalence, while rural areas had more pesticide-related impacts); Chemical and Biological Research (research identified effective natural remedies, such as Agaricus bisporus extract, and studied pesticide residues' effects on honey and beeswax); Behavioral and Genetic Studies (studies on disease transmission behaviors, genetic responses to stressors, and the influence of food diversity reveal how bees adapt to environmental challenges and human activities); and Global and Local Initiatives (efforts such as citizen science for monitoring wild bees, the development of biosecurity measures, and comparative studies on bee species in diverse habitats aim to mitigate risks and improve resilience).
Conventional to Emerging Advances in Plant Disease Diagnosis and Pathogen Detection
The book "Conventional to Emerging Advances in Plant Disease Diagnosis and Pathogen Detection" comprehensively addresses key diagnostic methods and pathogen detection techniques, from their origins to the present, guiding us toward the future of plant diagnostics.
The Evolution of Imperfection
How understanding our genetic imperfections can change our view of evolution and enrich what it means to be human If we start with the presumption that evolution is a constantly improving process, some aspects of our evolution just do not make sense. We have a high rate of genetic diseases, for example, and much of our DNA seems to be pointless. In The Evolution of Imperfection, Laurence Hurst explores our apparently rotten genetic luck. Hurst, a leading authority on evolution and genetics, argues that our evolutionary imperfections proceed directly from two features: the difficulties of pregnancy and the fact that historically there are relatively few of us. In pregnancy, natural selection can favor chromosomes that kill embryos in species (including ours) that continuously receive resources from the mother. Most fertilized eggs don't make it, and incompatibilities between the fetus and mother can lead to lethal disorders of pregnancy. The historically small population size enhances the role of chance, which in turn leads to both accumulation of unnecessary DNA and more mutation. So what can save us? One answer may lie in genetic medicine, which has given us therapies that make killer conditions preventable and even curable. Hurst suggests that our seeming imperfections could be the key to a new way to understand evolution itself. Looking at circumstances that seem to defy explanation, we might come to a richer understanding of how evolution really works, and what it means to be human.
Metalloids in Biology
Metalloids belong to class of elements that exhibit physiochemical characteristics intermediating between those of metals and non-metals. Some are quasi-essential for the overall growth and development of plants. This book is aimed to give updated and scientific insights to readers and researchers and environmentalist.
Visceral Leishmaniasis and Post-kala-azar Dermal Leishmaniasis
This reference book delves into visceral leishmaniasis (VL) and Post-Kala-Azar Dermal Leishmaniasis (PKDL), covering their pathophysiology, treatment options, and control strategies. It reviews VL's global impact, its transition to PKDL, symptoms, diagnosis, and the immunopathogenesis of both diseases, highlighting parasite-host interactions
Mathematical Properties of Population-Genetic Statistics
A powerful new approach to interpreting population-genetic data in evolution and ecology Population genetics uses statistical analysis to catalog genetic variation among populations and species. Summary statistics computed from allele frequencies--mathematical functions that measure features of genetic similarity and diversity--are key to this global effort. Yet despite their widespread use in evolutionary biology, ecology, and conservation biology, their mathematical properties have largely been overlooked. This book shows how to use the mathematical bounds on summary statistics to make better interpretations of population-genetic data. Noah Rosenberg discusses how the behavior of these statistics depends not only on the biology of the populations they seek to describe but also on the mathematical properties of the functions used to compute them, properties that produce constraints on the values of the statistics and influence their interpretation. Focusing on the concept of homozygosity--a quadratic function of allele frequencies in a population--he demonstrates how to account for mathematical constraints when measuring genetic similarity and diversity. Rosenberg illustrates the results using examples from empirical data and shares strategies that readers can use to apply this mathematical perspective to different kinds of summary statistics, including those for measuring biodiversity in ecological communities. Drawing inspiration from Charles Darwin, who marveled at evolution's "endless forms most beautiful and most wonderful," this book presents a groundbreaking approach to the study of genetic variation. It is sure to stimulate new research in population biology and deeper thinking about the meaning and interpretation of essential measurements of the world's genomes.
Mathematical Properties of Population-Genetic Statistics
A powerful new approach to interpreting population-genetic data in evolution and ecology Population genetics uses statistical analysis to catalog genetic variation among populations and species. Summary statistics computed from allele frequencies--mathematical functions that measure features of genetic similarity and diversity--are key to this global effort. Yet despite their widespread use in evolutionary biology, ecology, and conservation biology, their mathematical properties have largely been overlooked. This book shows how to use the mathematical bounds on summary statistics to make better interpretations of population-genetic data. Noah Rosenberg discusses how the behavior of these statistics depends not only on the biology of the populations they seek to describe but also on the mathematical properties of the functions used to compute them, properties that produce constraints on the values of the statistics and influence their interpretation. Focusing on the concept of homozygosity--a quadratic function of allele frequencies in a population--he demonstrates how to account for mathematical constraints when measuring genetic similarity and diversity. Rosenberg illustrates the results using examples from empirical data and shares strategies that readers can use to apply this mathematical perspective to different kinds of summary statistics, including those for measuring biodiversity in ecological communities. Drawing inspiration from Charles Darwin, who marveled at evolution's "endless forms most beautiful and most wonderful," this book presents a groundbreaking approach to the study of genetic variation. It is sure to stimulate new research in population biology and deeper thinking about the meaning and interpretation of essential measurements of the world's genomes.
Molecular Mechanisms of Bioactive Nutrients Promoting Health through Gut Microbiota 2.0
The present Special Issue of the International Journal of Molecular Sciences focuses on the relevant and rapidly developing topic of gut microbiota research. As the articles within this issue highlight, the human gut microbiota plays a critical role in human health. Dietary intake directly influences gut microbiota composition, and the resulting changes in gut metabolites can have profound effects on the host. This Special Issue brings together the latest findings on the intricate relationship between bioactive food components, gut microbiota, colon health, and chronic metabolic diseases. The gut microbiota is a complex ecosystem composed of trillions of microorganisms, including bacteria, archaea, fungi, and viruses. These microbes play essential roles in digestion, nutrient absorption, immune function, and even mental health. Importantly, the composition of the gut microbiota is not static; it is constantly changing in response to diet, lifestyle factors, and medications. The articles within this issue highlight the vast potential of dietary interventions to modulate gut microbiota composition and promote health. As our understanding of this complex ecosystem continues to grow, we can expect to see the development of novel therapeutic strategies for a wide range of chronic diseases.
Computational Biology
This extensively expanded third edition offers a practical introduction to Bio Data Science. With a hands-on approach to learning, this book offers ample opportunities to practice: - Installing and utilizing Linux as a virtual machine or remotely - Processing bio data with the programming language AWK - Managing data with the relational database system MariaDB - Analyzing and visualizing data with R - Implementing good bioinformatics practices with Jupyter Notebook and GitHub This book targets both students and professionals in the life sciences. While it is aimed at beginners, it also provides valuable tips and tricks for experienced researchers dealing with large datasets. Worked examples illustrate how to utilize various bioinformatics tools such as BLAST, Clustal, PLINK, IGV, SAMtools, BCFtools, Mason2, Minimap, NCBI Datasets, Velvet, Jmol, and more for: - Identifying bacterial proteins potentially associated with pathogenicity - Querying molecular structures for redox-regulated enzymes - Mapping and assembling real or simulated sequence reads - Identifying and mapping molecular structure mutations in viruses - Conducting genome-wide association studies All software tools and datasets mentioned are freely available, and all code is accessible as Jupyter Notebooks on GitHub. Drawing from the author's experiences and knowledge gained from both academia and industry, this book provides a practical and comprehensive approach to bioinformatics.
Preparation and Application of Advanced Functional Membranes
This reprint offers updated studies on functional membranes for strategic areas such as water cleaning and reuse, environmental remediation, drug delivery, energy recovery, and CO2 capture, but also the recycling of bio-based and ecofriendly materials for membrane manufacturing and purification processes that impact important domains of society and industry. Theoretical modelling, structure-transport assessment, and experimental findings contribute to creating a multidisciplinary representation of membrane science in its many facets. This reprint is a useful reference for inspiring scientists and students who want to tackle scientific and societal challenges within a sustainable and circular chemistry framework.
Introduction to Crispr-Cas9 Techniques
This open-access textbook provides an in-depth introduction into the CRISPR-cas9 technology and explores its use across the gamut of biological model systems. As the subject has risen from a significant new discovery to a mainstream molecular biology practice, it is essential that students of molecular biology understand the fundamentals behind CRISPR-Cas9 technology and how it may be employed efficiently and ethically in research. This volume, edited by experts in both, molecular biology and undergraduate education, will teach not only the fundamentals of using CRISPR-Cas9, but also how to successfully employ this technology in classroom settings. The book is written for undergraduates and advanced high school classes in the area of molecular biology, genetics, genomics and biological engineering and will provide a perfect tool for undergraduate lecturers to prepare their classes.
Antioxidant Activity of Honey Bee Products
The published scientific papers highlight the significance of utilizing the antioxidative properties found in various compounds of natural bee products. By exploring a diverse range of bioactive substances present in propolis, honey, pollen, cerumen, and other products, the authors are paving the way for sustainable beekeeping and apitherapeutic practices. Additionally, incorporating natural antioxidants into functional foods presents an innovative and promising opportunity to enhance humans' and animals' health and well-being.
Fisheries and Blue Economy
This reprint brings together a selection of papers on the role of fisheries and aquaculture in the blue economy, presenting research on the challenges and opportunities for developing fisheries and aquaculture under the sustainable blue economy concept. The results highlight options for developing the blue economy to enable the potential role of fisheries in Africa and Asia and elsewhere to realise benefits of blue economy development including food security and poverty reduction that can be realised through the sustainable development of fishery resources.
Effect of Antimicrobial Peptide LL-37 Against Pseudomonas areuginosa
Novel Nanomaterials and Nanotechnology
The aim of this special issue is to showcase significant advancements in biosensors, emphasizing the innovative use of nanomaterials and aptasensors for bio-detection and food safety. This Special Issue presents diverse studies, including optical differential sensing platforms, PCR-independent RNA detection biosensors, and cancer biomarker identification strategies. Key contributions emphasize the enhancement of sensitivity using nanomaterials such as mesoporous silica and Au@Ag nanorods, along with nucleic acid functional materials like DNA hydrogels. Additionally, the development of rapid and selective sensors for detecting pathogens and nutrients is highlighted. This collection serves to inspire further research in biosensing technologies to improve food safety and public health.
Advances in Oil and Gas Wellbore Integrity
Wellbore integrity is a key issue surrounding the life of oil and gas drilling, production, and abandonment. It focuses on advanced techniques applied to well integrity testing and assessment to achieve safe and efficient production. With the development of digitalization and intelligence in the oil and gas industry, new solutions are provided for well integrity monitoring. It is one of the innovative methods of oil and gas well integrity detection that can achieve the visualization analysis of big data through reliable signal analysis methods, such as wellbore flow parameters obtained by sensors and real-time casing monitoring. Therefore, the traditional and unconventional methods used to detect and assess well integrity are the focus of this issue. In particular, the processing method, calculation standard, and visual analysis of well monitoring data are used in well integrity assessments. Factors may include the reliability of wellbore flow models, effective information extraction from real-time wellbore monitoring data, and coupled evaluation models involving all barrier elements of wellbore integrity. This research can be based on practical experience in the field or through detailed simulations.
In the Arms of Morpheus
This reprint comprises ten chapters, with six original research articles and four review works on different aspects of dreaming, including the following: a) basic studies on perceptual experiences, lucid dreaming, and pre-sleep visual media exposure;b) clinical issues involving schizophrenia, depression, nightmares, and suicide attempts;and c) theoretical reviews regarding REM sleep controversies and the epistemic significance of impactful dreams.
Work Psychology and Occupational Health
This Special Issue focuses on topics such as the effect of the pandemic on the working environment; the relationship between the pandemic and the psychological effects on workers; and maintenance and/or measures for workers' health (especially mental health) during the pandemic. The key items for the above objectives are the introduction and increase of telework and online operations; changes in the working environment, such as reduced working hours, dismissal, and the effects of unemployment; changes such as increased stress and decreased coping strategies among workers; increased loneliness and isolation, as well as decreased opportunities for communication-including opportunities for employees to meet for mental health resources-and an increase in the need for self-care as a stress-coping method that can be practiced by one person. It also includes hands-on experience in dealing with the increase in serious stress and loneliness via strong support at the organizational and individual levels.
Advancements in Cardiovascular and Antidiabetic Drug Therapy
The aim of this reprint is to immerse the reader in the transformative advancements in drug-based therapies for cardiovascular diseases and diabetes, two major contributors to global morbidity and mortality. From the groundbreaking use of sodium-glucose cotransporter 2 inhibitors in heart failure and diabetes management to the development of novel incretin therapies and innovative insulin delivery systems, this collection examines the potential and promise of these cutting-edge pharmacological strategies.In this special issue, leading experts in the fields of cardiology and diabetology share their insights, clinical findings, and visions for the future of integrated therapeutic approaches. Together, we embark on a journey to redefine the prevention and treatment of cardiovascular and metabolic disorders, offering sustainable solutions to improve patient outcomes and quality of life. This Special Issue paves the way towards a healthier, more resilient future where the burden of these interconnected conditions is mitigated by science-driven innovations.
Oral Cancer
Oral cancer contributes to approximately 3-10% of all cancer mortality worldwide, and its incidence is continuously increasing due to environmental conditions and harmful modern lifestyles. In association with hereditary predisposition, chronic inflammation, and infectious diseases, these factors raise the frequency of oral cavity malignancies. Oral squamous cell carcinoma (OSCC) represents 90% of oral neoplasia; it is the sixth most frequent malignancy in the world, with an overall 5-year survival rate below 50% due to its modest outcomes, late diagnosis, aggressive local invasiveness, recurrences, and metastases. OSCC belongs to the comprehensive group of head and neck squamous cell carcinomas (HNSCCs), which affect the cell lining of the oral cavity, pharynx, and larynx. The local-regional recurrence rate for advanced carcinomas is over 50% despite the administration of multimodal therapy, and understanding the biology of HNSCCs is essential to ameliorate their prognosis. A major intervention involves extensive surgery associated with radio and chemotherapy. Advancements in cancer immunotherapy have not yet established the specific mechanisms by which immune cells influence tumor progression and immune evasion, and oral cancers continue to respond inadequately to the current treatment protocols. The above-mentioned aspects underline this Special Issue, which has brought together the most recent research on molecular mechanisms implied in the prophylaxis, etiopathogenesis, diagnosis, treatment, evolution, and prognosis of oral cancer.
Interdisciplinary Research into Cultural and Biological Transformations in the Paleolithic Period
The incredibly long Paleolithic period is still considered by some to be a stagnant phase in human cultural and biological evolution prior to the appearance of our direct ancestors. However, in recent years, extensive interdisciplinary research has clearly demonstrated that this is not the case. Starting from the earliest stages of human presence on the planet some three million years ago, an impressive series of transformations, innovations, modifications and adaptations characterize our lineage. These changes in behavior and culture took place alongside biological adaptations in human physical properties, faunal turnovers and extinctions, as well as climatic fluctuations. This makes the Old Stone Age a hectic, dynamic and lively epoch that is worthy of investigation, both at the diachronic and synchronic levels, in order to decipher the nature of the transformations that characterize the emergence, prosperity and legacy of our lineage. This Special Issue, titled "Interdisciplinary Research into Cultural and Biological Transformations in the Paleolithic Period", presents state-of-the-art, as well as outside-of-the-box, studies regarding changes in human adaptation, human physiology, faunal diversity and climatic fluctuations, as well as the possible nexus between these lines of inquiry.
Natural Bioactive Compounds Exerting Health Promoting Effects through Ameliorating Oxidative Stress
Oxidative stress is caused by various intrinsic and extrinsic factors. It has been widely accepted that dietary or dietary supplement interventions can decrease levels of oxidative stress. Natural bioactive compounds (flavonoids, stilbenes, terpenes, alkaloids, saponins, polysaccharides, etc.) have been reported to exhibit multiple health-promoting effects, including antioxidant activity. However, the underlying cellular signaling and molecular mechanisms of their effect in ameliorating oxidative stress are not fully understood. In this Special Issue, we have collected and summarized the antioxidant activities of natural bioactive compounds based on the studies from cell culture models, animal models, and clinical trials. This Special Issue contains twelve papers covering the above-mentioned topics, including one systematic review and meta-analysis, one editorial, and ten research papers.
High resolution melting assay for detection STD bacterial infections
Synthetic Biology (2nd Ed)
This first edition of this book was the first manual for laboratory work in the rapidly expanding field of synthetic biology. Aimed at non-specialists, it contained protocols central to synthetic biology in both research and education. It also included information for students of the annual international Genetically Engineered Machine (iGEM) competition and the emerging biohacker community. In addition, it detailed a colorful lab course in bacterial synthetic biology based on chromoproteins. The second edition covers new material including our next-generation less-toxic versions of the chromoproteins (now made available as kits), UV-free imaging and quantitation with smartphones, a computational modeling lab and yeast experiment, logic gates, type IIS cloning and the CRISPR revolution.Our decade-old manual is still the only lab course book in synthetic biology that is based on chromoproteins, the simplest bacterial reporters. However, this has become outdated due to our development of next-generation, less-toxic chromoproteins and UV-free quantitation. The main competing lab course book in synthetic biology is still the decade-old BioBuilder: Synthetic Biology in The Lab by Kuldell et al. (2015). However, it is based on outdated approaches that use more complicated methods than chromoproteins to enable colored reporters, making it a less-suitable educational tool. In addition, topical issues in synthetic biology such as computational modeling, logic gates, type IIS cloning and eukaryotic experiments, including the CRISPR revolution are now covered.
Morphology of the Digestive Tract of the Fish Steindachnerina notonota
Synthetic Biology (2nd Ed)
This first edition of this book was the first manual for laboratory work in the rapidly expanding field of synthetic biology. Aimed at non-specialists, it contained protocols central to synthetic biology in both research and education. It also included information for students of the annual international Genetically Engineered Machine (iGEM) competition and the emerging biohacker community. In addition, it detailed a colorful lab course in bacterial synthetic biology based on chromoproteins. The second edition covers new material including our next-generation less-toxic versions of the chromoproteins (now made available as kits), UV-free imaging and quantitation with smartphones, a computational modeling lab and yeast experiment, logic gates, type IIS cloning and the CRISPR revolution.Our decade-old manual is still the only lab course book in synthetic biology that is based on chromoproteins, the simplest bacterial reporters. However, this has become outdated due to our development of next-generation, less-toxic chromoproteins and UV-free quantitation. The main competing lab course book in synthetic biology is still the decade-old BioBuilder: Synthetic Biology in The Lab by Kuldell et al. (2015). However, it is based on outdated approaches that use more complicated methods than chromoproteins to enable colored reporters, making it a less-suitable educational tool. In addition, topical issues in synthetic biology such as computational modeling, logic gates, type IIS cloning and eukaryotic experiments, including the CRISPR revolution are now covered.
Alive
A profound and provocative journey through the human body from a surgeon and award-winning writer. "Weston's evocative descriptions will change how readers see the body....This captivates."--Publishers Weekly (starred review) What does it mean to live in a body? For Gabriel Weston, there was always something missing from the anatomy she was taught at medical school. She'd forged an unconventional path, first studying humanities and getting an entry-level job in publishing, before a spark of inspiration set her on the path to becoming a doctor. Medicine teaches us how a body functions, but it doesn't help us navigate the reality of living in one. As Weston became a surgeon, a mother, and ultimately a patient herself, she found herself grappling with the gap between scientific knowledge and unfathomable complexity of human experience. In this captivating exploration of the body, Weston dissolves the boundaries that usually divide surgeon and patient, pushing beyond the limit of what science has to tell us about who we are and leaning on her roots in the humanities. Focusing on our individual organs, not just under the intense spotlight of the operating theatre, but in the central role they play in the stories of our lives, a fuller and more human picture of our bodies emerges: more fragile, frightening and miraculous than we could have imagined. Intimate, penetrating and original, Alive is about our bodies and bonds, the richness and brevity of existence, and the thread of mortality that connect us all.
